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    Evidence summary

    Population and studies description

    This interventional procedures overview is based on about 3,000 people from 4 randomised controlled trials (Di Costanzo 2015, Orlacchio 2014, Ferrari 2007, Zou 2017), 1 quasi-randomised controlled trial (Ferrari 2006), 1 retrospective non-randomised comparative study (Adwan 2022), 1 case-control study (Morisco 2018), 5 cohort studies (Pacella 2009, Francica 2012, Vogl 2004, Vogl 2014, Vogl 2013) and 1 case report (Helmberger 2002). Of these 3,000 people, about 2,500 had the procedure. There is likely to be some population overlap between the studies. This is a rapid review of the literature, and a flow chart of the complete selection process is shown in figure 1. This overview presents 13 studies as the key evidence in table 2 and table 3, and lists 40 other relevant studies in table 5.

    Most of the studies were from Italy or Germany, with 1 randomised controlled trial from China. All 4 randomised controlled trials included people with HCC and compared laser ablation with RFA. The non-randomised studies included people with HCC and metastatic liver tumours, and comparators included microwave ablation, TACE, and percutaneous ethanol injection. Most of the studies included people with up to 5 small tumours (maximum diameters ranged from 3 cm to 5 cm) and with Child-Pugh class A or B cirrhosis. An exception was the case control study by Morisco (2018), which compared laser ablation with TACE in a population with a solitary HCC 40 mm or larger. The retrospective cohort study by Francica (2012) aimed to compare outcomes of laser ablation in HCC tumours at high-risk locations compared with those at standard risk locations. Several studies reported follow up beyond 5 years.

    In the study by Ferrari (2006), which has been described as quasi-randomised, computer randomisation was used for most treatments but there were some exceptions. Tumours with a difficult percutaneous approach were treated with TACE, and an alternative treatment to laser ablation was used for superficial lesions next to large vessels and cholecysts.

    The cohort study by Vogl (2014) specifically stated that 57% (n=337) of people had treatment with a curative intention aiming to eliminate disease from the liver and 43% (n=257) had treatment with a palliative intention.

    Table 2 presents study details.

    Figure 1 Flow chart of study selection

    Table 2 Study details

    Study no.

    First author, date

    country

    Patients (men: women)

    Age (years)

    Study design

    Inclusion criteria

    Intervention

    Follow up

    1

    Di Costanzo G, 2015

    Italy

    n=140 (157 tumours)

    100:40

    HCC

    Median 70 (range 36 to 84)

    Non-inferiority randomised controlled trial (single centre)

    January 2009 to September 2012

    Patients with cirrhosis and unresectable HCC or who refused surgery; solitary HCC 5 cm or smaller, or 1 to 3 tumours each 3 cm or smaller in diameter; Child-Pugh class A or B; platelet count above 40,000 per microlitre and INR below 2.0; no previous HCC treatment.

    • Laser ablation, with Echolaser (Elesta, Italy) using 1 to 4 fibres (n=70 patients, 80 tumours)

    • RFA, using a single electrode (n=70 patients, 77 tumours)

    All procedures were done under ultrasound guidance.

    Up to 59 months

    2

    Orlacchio A, 2014

    Italy

    n=30 (30 tumours)

    21:9

    HCC

    Mean 72.4 (range 52 to 78)

    Randomised controlled trial (single centre)

    2009 to 2011

    Patients with cirrhosis and single HCC tumour with maximum diameter 40 mm; Child-Pugh class A or B.

    • Laser ablation, with Echolaser XVG system, using 2 to 4 fibres (n=15)

    • RFA, using a single expandable electrode (n=15)

    All procedures were done under combined ultrasound and CT guidance.

    Lesions that showed a partial response at 30 days were retreated with the same technique.

    Up to 12 months

    3

    Ferrari F, 2007

    Italy

    n=81 (95 tumours)

    56: 25

    HCC

    Mean 69.3 (range 51 to 82)

    Randomised controlled trial

    January 2003 to December 2005

    Patients with cirrhosis and a single HCC tumour with a maximum diameter of 40 mm or with a maximum of 3 HCC tumours each measuring no more than 30 mm in diameter and who had not had previous treatment for HCC; performance status between 0 and 2, cardiac and pulmonary function between 0 and 2 (World Health Organization) and age ranging from 19 to 82 years. Prothrombin time less than 40% (INR greater than 1.99) or platelet count less than 40,000/ml caused treatment to be delayed until adequate values had been restored in 22 patients. Patients with evidence of vascular tumour extension, invasion of the main bile duct or extrahepatic metastases on CT were excluded.

    • Laser ablation using a Nd-YAG laser (DEKA-M.E.L.A, Italy); n=41, 66 treatment sessions. Up to 4 fibres were positioned inside the tumour.

    • RFA, using single or cluster closed tip, internally cooled electrodes, n=40.

    Additional treatment sessions were offered if ablation was incomplete after the initial session.

    Both procedures were done under ultrasound guidance.

    Not reported

    4

    Zou D, 2017

    China

    n=74 (69 had a single tumour)

    58: 16

    HCC

    Range 37 to 76

    Randomised controlled trial

    October 2013 to March 2016

    HCC and increased alphafetoprotein; diameter of single tumour foci, or the sum of diameters of 2 adjacent tumour foci was less than 3 cm, without infiltration of blood vessels and bile ducts, abdominal lymph nodes or distant transfer; patients chose laser ablation or RFA as the first treatment and had no previous treatment history; Child-Pugh of liver function was A or B level.

    • Laser ablation using EchoLaser X4 system (ESAOTE S.P.A, Italy); n=35

    1 to 3 needles were placed under ultrasound guidance and the procedure was monitored continuously with real-time ultrasound guidance.

    • RFA using 1 or 2 electrode needles; n=39

    3 months

    5

    Ferrari F, 2006

    Italy

    n=131 (148 tumours)

    94:37

    HCC

    1 patient was waiting for a liver transplant.

    Mean 67.8 (range 44 to 82)

    Quasi-randomised controlled trial (mostly randomised with some exceptions)

    November 1998 to March 2004

    Single HCC tumour with maximum diameter of 40 mm or multiple tumours (maximum 3) each with a diameter of 30 mm or smaller; Child-Pugh class A or B, Patient Performance Status of 0 to 2, cardiac and lung function of 0 to 2 (World Health Organisation) and age between 18 and 80.

    • Laser ablation under ultrasound guidance, with a multifrequency convex probe with a lateral needle guide system (n=46, 50 tumours)

    • TACE (n=18, 20 tumours)

    • PEI under ultrasound guidance (n=34, 38 tumours)

    • Combined TACE and PEI (n=33, 40 tumours)

    Up to 84 months

    6

    Adwan H, 2022

    Germany

    n=303 (510 tumours)

    239:64

    HCC

    Mean 67.5 (laser ablation), 66 (microwave ablation)

    Retrospective non-randomised comparative study

    Early or intermediate tumour stage with HCC maximum axial diameter 5 cm, maximum 5 tumours, and adequate coagulation (INR 1.5 or less, or thrombocytes 50,000 per microlitre or more).

    • Laser ablation under MRI guidance (n=53, 75 tumours, 76 sessions)

    Nd-YAG laser, Avanto, Siemens, using SOMATEX laser application kit. Puncture of the tumour and insertion of the laser applicator was done under CT guidance.

    • Microwave ablation under CT guidance (n=250, 435 tumours 445 sessions). CT fluoroscopic scans were repeatedly done to monitor ablation.

    Mean 35.7 months (laser), 31.5 months (microwave)

    7

    Pacella C, 2009

    Italy

    n=432 (548 tumours)

    278:154

    HCC

    52 people were potentially eligible for liver transplant.

    Mean 67.7

    Retrospective (from 1994 to January 2004) and prospective (from February 2004 onwards) multicentre cohort study

    1994 to 2005

    Cirrhosis and early HCC (single tumour 4 cm or less or 1 to 3 tumours, each 3 cm or less in diameter) and surgery was not an option.

    Exclusion criteria included extrahepatic metastases; local, segmental, or lobar portal venous thrombosis; uncontrolled liver disease decompensation; severe clotting impairment; renal failure; or Child-Turcotte-Pugh class C cirrhosis.

    Laser ablation using a Nd:YAG, laser (DEKA-M.E.L.A., Italy) and an optical beam-splitting device (SMART 1064 HCC; DEKA-M.E.L.A.) with 4 separate fibres. One to 4 needles were placed under ultrasound guidance and the procedure was monitored continuously with real-time ultrasound guidance.

    Median 36 months (reverse Kaplan-Meier estimate), range 1 to 109 months.

    8

    Morisco F, 2018

    Italy

    n=82 (82 tumours)

    58: 24

    HCC

    Median 72 (range 49 to 88)

    Retrospective case control study (historical control group)

    January 2009 to December 2012

    Unresectable HCC or refusal of surgery; solitary HCC 40 mm or larger; Barcelona Clinic Liver Cancer stage A or B; Child–Pugh class A or B cirrhosis; platelet count more than 40,000 per microlitre and INR less than 2.0; no history of previous HCC treatment.

    Exclusion criteria included: history of encephalopathy or refractory ascites; vascular invasion or extrahepatic metastasis; severe comorbidities reducing life expectancy.

    • Laser ablation using 4 or 8 fibres (n=41); 25 patients had 2 treatment sessions and 7 had 3 sessions.

    • TACE (n=41); all patients had at least 2 treatment sessions

    Mean 37.4 months

    9

    Francica G, 2012

    Italy

    n=164 (182 tumours)

    90:74

    HCC

    n=106 with tumour at high risk location, 58 at standard risk location

    Mean 68.6 (range 42 to 84)

    Retrospective cohort study

    January 1996 to June 2008

    Single HCC tumour 4 cm or less in diameter or multiple tumours (no more than 3) each 3 cm or less in diameter irrespective of location; Child-Pugh class A or B cirrhosis; lack of extrahepatic metastases and lack of local, segmental, or lobar portal venous thrombosis; and follow-up of at least 6 months. A high-risk location was defined as less than 5 mm from large vessels or vital structures.

    Laser ablation with Nd:YAG laser (279 sessions)

    2, 3 or 4 optic fibres advanced in needles were positioned under ultrasound guidance and the procedure was monitored continuously with real-time ultrasound guidance.

    Median 81 months (range 6 to 144)

    10

    Vogl T, 2004

    Germany

    n=603

    (1,801 tumours)

    432: 171

    CRC liver metastases

    Mean 61.2 (range 31 to 89)

    Cohort study

    June 1993 to February 2002

    173 patients had already had treatment as part of earlier clinical trials.

    Recurrent liver metastases after partial liver resection, metastases in both liver lobes, locally nonresectable tumours, general contraindications for surgery or refusal to have surgical resection.

    Exclusion criteria included more than 5 tumours larger than 5 cm in greatest diameter or known extrahepatic tumour spread.

    Laser ablation using non-irrigated laser application system with limited power settings (n=56 [group 1]), or internally cooled laser application system (n=117 already treated [group 2] and n=430 prospectively treated [group 3]); 1,555 treatment sessions.

    Group 3 had more aggressive treatment than group 2, with more applicators per tumour. The needles were placed under CT guidance and MRI was used to monitor the procedure.

    Up to 7.6 years

    11

    Vogl T, 2014

    Germany

    n=594

    (1,545 tumours)

    406: 188

    CRC liver metastases

    Mean 61.2 (range 25 to 87)

    Single centre cohort study

    January 1999 to December 2010

    Recurrent liver metastases after partial liver resection, metastases in both liver lobes, locally nonresectable tumours, general contraindications for surgery or refusal to have surgical resection, maximum of 5 tumours, tumour size 5 cm or less in diameter.

    MRI-guided laser ablation using a Nd:YAG laser (Dornier MedTech, Germany); mean number of sessions per patient=2.26 (range 1 to 7).

    Needle puncture was done with CT guidance. For large tumours, multiple applicators were used.

    Mean 22.5 months (range 0 to 121)

    12

    Vogl T, 2013

    Germany

    n=401

    (809 tumours)

    58: 343

    Liver metastases from primary sites other than CRC (mainly breast)

    Mean 57.3 (range 26 to 86)

    Cohort study

    January 2000 to January 2011

    Maximum of 5 tumours, metastases of 5 cm or less in diameter, recurrent liver metastases after partial liver resection, metastases in both liver lobes, and general contraindications for surgery.

    Exclusion criteria: CRC liver metastases, poor general condition (Karnofsky status less than 70%).

    MRI-guided laser ablation using a Nd:YAG laser (Dornier MedTech, Germany); 736 treatment sessions.

    Needle puncture was done with CT guidance. For large tumours, multiple applicators were used. The mean and median number of applicators per tumour was 2 (range 1 to 5).

    Not reported

    13

    Helmberger T, 2002

    n=1

    male

    Liver metastases (history of renal cell carcinoma)

    57

    Case report

    The patient had 5 liver metastases (maximum diameter 5 cm), in both liver lobes and located in central hepatic areas, surgical resection was not considered.

    Laser ablation using a Nd:YAG laser (Dornier MedTech, Germany). An introducer sheath was placed into each tumour under CT guidance, to direct the laser fibre into the tumour. The position of the laser fibre was controlled by CT fluoroscopy for each placement during and at the end of the ablation process.

    Perioperative

    Table 3 Study outcomes

    First author, date

    Efficacy outcomes

    Safety outcomes

    Di Costanzo, 2015

    Complete tumour ablation (absence of any contrast enhancement within or at the periphery of the HCC nodule, assessed by CT or MRI):

    • Laser ablation=95.7% (67 out of 70; 95% CI 88.1 to 98.5) of patients and 96.3% (77 out of 80; 95% CI 89.6 to 98.7) of nodules

    • RFA=97.1% (68 out of 70; 95% CI 90.2 to 99.2) of patients and 97.4% (75 out of 77; 95% CI 91.0 to 99.3) of nodules

    Overall treatment sessions to obtain complete tumour ablation:

    • Laser ablation=82

    • RFA=72, p=0.058

    4 people had liver transplant (3 laser ablation and 1 RFA). Evaluation of explanted livers showed complete necrosis of treated nodules.

    Local tumour progression:

    • Laser ablation=22.9% (16 out of 70)

    • RFA=25.7% (18 out of 70), p=0.956

    Mean time to local progression (months):

    • Laser ablation=45.9 (95% CI 40.6 to 51.2)

    • RFA=39.6 (95% CI 34.1 to 45.0), p=0.376

    Note: There are some discrepancies in the reported results and the numbers above were taken from Table 2 of the publication.

    Mean overall survival (months):

    • Laser ablation=42.2 (95% CI 37.2 to 47.2)

    • RFA=42.8 (95% CI 38.3 to 47.3), p=0.346

    1-year survival probability:

    • Laser ablation=94%

    • RFA=94%

    3-year survival probability:

    • Laser ablation=80%

    • RFA=89%

    Mortality during study period:

    • Laser ablation=34.3% (7 cancer progression, 10 liver failure, 2 cancer progression and liver failure, 2 variceal bleeding, 3 other causes)

    • RFA=26% (6 cancer progression, 3 liver failure, 5 cancer progression and liver failure, 1 variceal bleeding, 3 other causes)

    There were no treatment-related deaths.

    Moderate pain (SIR Class A):

    • Laser ablation=33%

    • RFA=36%

    Self-limiting fever lasting less than 15 days (SIR Class A):

    • Laser ablation=35%

    • RFA=32%

    Subcutaneous tumour seeding (SIR Class C):

    • Laser ablation=1.4% (1 out of 70)

    • RFA=1.4% (1 out of 70)

    In both cases, it was surgically removed.

    Orlacchio, 2014

    Technical success=100%

    Complete response at 30 days (assessed by CT, evaluated according to the mRECIST criteria):

    • Laser ablation=66.7% (10 out of 15)

    • RFA=86.7% (13 out of 15)

    Complete response after second treatment session:

    • Laser ablation=86.7% (13 out of 15)

    • RFA=93.3% (14 out of 15), p=not significant

    Number of treatment sessions per tumour needed to obtain complete ablation:

    • Laser ablation=1.38

    • RFA=1.21

    The 3 tumours that did not show a complete response were treated with chemoembolisation.

    No patients died during the first 12 months of follow up.

    Cumulative rates of freedom from local disease progression:

    • 3 months: laser ablation=85%, RFA=92%

    • 6 months: laser ablation=62%, RFA=86%

    • 12 months: laser ablation=54%, RFA=86%, p=0.083

    The cumulative rates of freedom from local disease progression at 12 months were higher for patients with tumours 20 mm or less in diameter.

    For tumours 21 mm or more in diameter, there was a higher rate of recurrence in the laser ablation group compared with RFA (p=0.0081).

    There were no major complications and no procedure-related deaths.

    Minor complications

    Asymptomatic perihepatic fluid collection:

    • Laser ablation, n=1

    • RFA, n=3

    Subcapsular haematoma:

    • Laser ablation, n=0

    • RFA, n=1

    Asymptomatic pleural effusion:

    • Laser ablation, n=1

    • RFA, n=4

    Side effects

    Vasovagal reaction:

    • Laser ablation, n=1

    • RFA, n=2

    Postablation syndrome:

    • Laser ablation, n=1

    • RFA, n=12

    Ferrari, 2007

    Complete tumour ablation (assessed by CT, defined as no contrast uptake seen in the ablation zone during the early arterial phase):

    • Laser ablation=78% (35 out of 45); 10 additional sessions

    • RFA=94% (47 out of 50); 3 additional sessions

    Disease recurrence (local tumour progression or new HCC tumour):

    • Laser ablation=26.8% (11 out of 41)

    • RFA=22.5% (9 out of 40)

    Of the 15 cases of local tumour progression, 60% were diagnosed at 12 to 24 months after treatment and the remaining 40% within the first 12 months; no local tumour progression was observed later than 24 months after treatment.

    Disease-free interval:

    • Laser ablation=15.45 months (SD 7.99)

    • RFA=17.78 months (SD 8.52)

    Cumulative survival rates

    • 12 months: Laser=88.6%, RFA=92.2%

    • 24 months: Laser=70.4%, RFA=75.0%

    • 36 months: Laser=56.6%, RFA=61.3%

    • 48 months: Laser=40.2%, RFA=54.6%

    • 60 months: Laser=22.9%, RFA=40.9% (at 55 months)

    p=0.3299

    Univariate analysis of survival revealed statistically significant differences between the Child-Pugh A and B groups (p<0.0001), between HCC nodules measuring 25 mm or less and more than 25 mm (p=0.0001) and between patients with a single tumour and with 2 tumours (p=0.0484).

    The Cox model showed that Child-Pugh class was the most important prognostic survival factor.

    No deaths or major or minor complications occurred during the procedures, and no cases of neoplastic seeding have been observed to date.

    Zou, 2017

    Complete response at 3 months (according to mRECIST criteria):

    • Laser ablation=88.6% (31 out of 35)

    • RFA=92.3% (36 out of 39), p=0.583

    Alphafetoprotein levels 3 months after treatment (µg/l):

    • Laser ablation=770.36 (baseline 973.67, p<0.001)

    • RFA=781.52 (baseline 983.78, p<0.001)

    The difference between the groups was not statistically significant.

    Carcinoembryonic antigen levels 3 months after treatment (U/l):

    • Laser ablation=182.68 (baseline 289.81, p<0.001)

    • RFA=180.65 (baseline 282.10, p<0.001)

    The difference between the groups was not statistically significant.

    Proportion of patients who reported great or general satisfaction 3 days after treatment:

    • Laser ablation=94.3% (33 out of 35)

    • RFA=69.2% (27 out of 39), p=0.022

    Adverse reactions

    Fever

    • Laser=11.4% (4 out of 35)

    • RFA=12.8% (5 out of 39), p=0.855

    Nausea

    • Laser=48.6% (17 out of 35)

    • RFA=48.7% (19 out of 39), p=0.990

    Vomiting

    • Laser=28.6% (10 out of 35)

    • RFA=30.8% (12 out of 39), p=0.836

    Diarrhoea

    • Laser=2.9% (1 out of 35)

    • RFA=2.6% (1 out of 39), p=0.522

    Abdominal pain

    • Laser=74.3% (26 out of 35)

    • RFA=74.4% (29 out of 39), p=0.994

    Skin rash

    • Laser=2.9% (1 out of 35)

    • RFA=7.7% (3 out of 39), p=0.687

    Ferrari, 2006

    Complete necrosis after first treatment session (assessed by CT, defined as no contrast impregnation in the tumour site in the early arterial phase (25 to 30 seconds after contrast injection):

    • Laser ablation=92% (46 out of 50) of tumours

    • TACE=70% (14 out of 20) of tumours

    • PEI=74% (28 out of 38) of tumours

    • Combined TACE and PEI=80% (32 out of 40) of tumours

    Further treatment sessions were offered after partial responses.

    Mean number of treatments per tumour:

    • Laser ablation=1.2, TACE=1.3, PEI=2.8, Combined TACE and PEI=5.3

    Recurrence-free interval (months):

    • Laser ablation=13.7 (8.7)

    • TACE=18.0 (SD 5.5)

    • PEI=27.0 (SD 13.5)

    • Combined TACE and PEI=13.9 (SD 8.9)

    • Total=15.7 (SD 9.4)

    Disease-free interval (months):

    • Laser ablation=14.3 (SD 8.0)

    • TACE=18.0 (SD 5.5)

    • PEI=29.8 (SD 13.4)

    • Combined TACE and PEI=15.4 (SD 16.1)

    • Total=17.0 (SD 13.7)

    Cumulative survival at 12, 36 and 60 months (%):

    • Laser ablation=90.0, 53.3, 31.0

    • TACE=66.7, 11.1, 0

    • PEI=71.0, 29.4, 18.4

    • Combined TACE and PEI=90.8, 41.4, 19.3

    • Total=81.9, 35.7, 20.8

    The survival rate for Child–Pugh class A was statistically significantly higher (p<0.0001) than for Child–Pugh class B.

    Those who had laser ablation or combined therapy survived longer than those who had TACE (p=0.0001 and 0.0096, respectively). Laser ablation was associated with longer survival than PEI (p=0.0274).

    There were no deaths or major complications during the procedures.

    There were 2 minor complications after TACE (cholecystitis). Side-effects of TACE included nausea, mild pain in the right hypochondrium and fever in the hours immediately following treatment. Severity of symptoms was directly proportional to the volume of the treated tumour.

    There were no cases of neoplastic cellular seeding in patients who had treatment with either PEI or laser ablation.

    Adwan, 2022

    Mean diameter of ablation area (cm):

    • Laser ablation=5.3

    • Microwave ablation=4.4, p=0.0001

    Technical success:

    • Laser ablation=100% (76 out of 76)

    • Microwave ablation=100% (445 out of 445)

    Initial complete ablation (assessed using contrast enhanced MRI):

    • Laser ablation=98.7% (74 out of 75)

    • Microwave ablation=97.7% (425 out of 435)

    Local tumour progression (defined as a new HCC tumour directly adjacent to the ablation area or an increase in the size of the ablation area at the follow-up):

    • Laser ablation=3.8% (2 out of 53)

    • Microwave ablation=6.0% (15 out of 250)

    Intrahepatic distant recurrence:

    • Laser ablation=64.2% (34 out of 53)

    • Microwave ablation=46.0% (115 out of 250)

    Overall survival at 1, 3 and 5 years from date of diagnosis:

    • Laser ablation=96.2%, 54.7%, 30.2%

    • Microwave ablation=94.3%, 65.4%, 49.1%, p=0.002

    Overall survival at 1, 3 and 5 years from ablation date:

    • Laser ablation=85.0%, 37.7%, 17.0%

    • Microwave ablation=86.6%, 53.4%, 40.4%, p=0.001

    Disease-free survival at 1, 3 and 5 years from ablation date:

    • Laser ablation=54.7%, 30.2%, 17.0%

    • Microwave ablation=45.9%, 30.6%, 24.8%, p=0.719

    Local tumour progression-free survival at 1, 3 and 5 years from ablation date:

    • Laser ablation=96.2%, 96.2%, 96.2%

    • Microwave ablation=95.2%, 93.8%, 93.8%, p=not significant

    Intrahepatic distant recurrence-free survival at 1, 3 and 5 years from ablation date:

    • Laser ablation=64.2%, 49.0%, 39.6%

    • Microwave ablation=55.6%, 46.4%, 42.4%, p=not significant

    There were no procedure related deaths in either group.

    Overall complication rate:

    • Laser ablation=7.9% (6 out of 76)

    • Microwave ablation=2.9% (13 out of 445), p=0.045

    There was 1 major complication, which was in the microwave ablation group (haemorrhagic pleural effusion, treated with thoracic drainage).

    Mild haemorrhage:

    • Laser ablation=2.6% (2 out of 76)

    • Microwave ablation=1.4% (6 out of 445)

    Pleural effusion:

    • Laser ablation=5.3% (4 out of 76)

    • Microwave ablation=1.4% (6 out of 445)

    Pacella, 2009

    Initial complete response (assessed by CT, according to modified World Health Organization criteria, classified as no areas of enhancement within or at the periphery of the ablation zone)= 78.2% (338 out of 432) of patients, 79.6% (436 out of 548) of tumours

    Complete ablation by tumour size

    • 2 cm or smaller=85.1% (183 out of 215)

    • Between 2.1 and 3.0 cm=81.8% (198 out of 242)

    • Between 3.1 and 4.0 cm=60.4% (55 out of 91)

    The probability of achieving a complete tumour ablation was statistically significantly greater in patients with a single tumour compared with patients with multiple tumours (p=0.015).

    Survival

    At the time of the analysis, 9 patients (2.1%) had had liver transplantations and 7 patients (1.6%) had been lost to follow up. 43.5% (188/432) of patients had died. Causes of death included HCC (n=88), extrahepatic metastases (n=11), and underlying cirrhosis and liver failure (n=56).

    Median overall survival=47 months (95% CI 41 to 53)

    • Child-Turcotte-Pugh class A=53 months (95% CI 44 to 62)

    • Child-Turcotte-Pugh class B=40 months (95% CI 33 to 47), p=0.088

    3-year cumulative survival=61%

    5-year cumulative survival=34%

    Multivariate Cox analysis showed that the independent predictors of survival were serum albumin level greater than 3.5 g/dl (p=0.002), complete tumour ablation (p=0.001), and age less than 73 years (p=0.001).

    Recurrence and disease-free survival

    Tumour recurrence (local and distant)=20% (67 out of 338)

    Median time to recurrence=24 months (95% CI 20 to 28)

    Median disease-free survival time=26 months (95% CI 22 to 30)

    Complications

    • Treatment-related death, n=1 (4 days after the procedure, the patient had acute liver decompensation associated with severe respiratory failure; also reported in Francica et al., 2012)

    • Major complications needing specific treatment=1.6% (7 out of 432)

    • Minor complications=98.1% (424 out of 432; most commonly asymptomatic pleural effusion [n=265])

    Major complications within 24 hours of procedure

    • Pancreatitis=0.2% (1 out of 432)

    • Intrahepatic haematoma=0.2% (1 out of 432)

    • Peritoneal bleeding=0.2% (1 out of 432)

    Major complications within 30 daysof procedure

    • Transient decompensation of liver function (SIR class C)=0.5% (2 out of 432)

    • Transient decompensation of liver function (SIR class D)=0.5% (2 out of 432)

    • Acute liver decompensation (SIR class F)=0.2% (1 out of 432)

    Morisco, 2018

    Complete response (according to the modified Response

    Evaluation Criteria in Solid Tumours):

    • Laser ablation=63.4% (21 out of 41)

    • TACE=19.5% (8 out of 41), p<0.001

    Response rate in tumours with diameter between 51 and 60 mm:

    • Laser ablation=75%

    • TACE=14.3%, p=0.013

    Disease recurrence in successfully patients:

    • Laser ablation=19.2% (5 out of 26)

    • TACE=75% (6 out of 8), p<0.0001

    Mean time to recurrence:

    • Laser ablation=42.2 months (95% CI 34.3 to 50.1)

    • TACE=26.8 months (95% CI 18.6 to 34.9), p=0004

    Mean disease-free survival:

    • Laser ablation=31.5 months (95% CI 24.1 to 38.8)

    • TACE=14.2 months (95% CI 9.7 to 18.7), p<0.0001

    Mean overall survival:

    • Laser ablation=39.7 months (95% CI 33.1 to 46.4)

    • TACE=37.0 months (95% CI 30.7 to 43.3), p=0.725

    Overall survival probability rates:

    • 1 year: laser ablation=90.2%, TACE=85.4%

    • 2 years: laser ablation=65.5%, TACE=65.9%

    • 3 years: laser ablation=55.4%, TACE=48.8%

    During the study period 50 patients died; 24 patients in the laser ablation group (12 HCC progression, 6 liver failure and 6 unknown causes) and 26 in the TACE group (16 HCC progression, 9 liver failure and 1 unknown cause).

    No safety outcomes were reported.

    Francica, 2012

    Complete ablation (assessed by CT, defined as no areas of enhancement at the periphery of the target area) = 93.4% (170 out of 182) of tumours

    Mean number of ablation sessions per tumour=1.58 (range 1 to 3)

    Complete response=92.7% (152 out of 164) of patients

    • High-risk location group=91.5% (97 out of 106) of patients, 92.2% (107 out of 116) of tumours

    • Standard risk location group=94.8% (55 out of 58) of patients, 95.5% (63 out of 66) of tumours, p=0.271 between groups

    Tumour size did not affect the probability of complete response.

    Local tumour progression=10.6% (18/170) of tumours

    There was no statistically significant difference in the cumulative incidence of local tumour progression among tumours at high-risk sites compared with elsewhere (p=0.499).

    Among the variables possibly associated with local tumour progression, only tumour size showed a trend at multivariate analysis (p=0.056) for an association to local recurrence.

    There was no statistically significant difference between the risk groups in terms of local tumour progression-free survival.

    Further treatment

    Twelve of the 18 tumours with local progression had further treatment: 5 had TACE and 7 had laser ablation; complete ablation was achieved in all those treated with laser ablation.

    Periprocedural major complications (within 30 days; per patient):

    • Death=0.6% (1 out of 164); the patient died 4 days after the laser ablation procedure of acute liver decompensation associated with severe respiratory failure (also reported in Pacella et al., 2009).

    • Main bile duct injury=0.6% (1 out of 164)

    These were both in the high-risk group.

    Minor complications (per session):

    • Effusion beneath Glisson capsule=1.8% (5 out of 279)

    • Subcapsular hematoma=0.4% (1 out of 279)

    • Partial necrosis of contiguous kidney=0.4% (1 out of 279)

    • Ascites=1.1% (3 out of 279; 1 was in the high risk group)

    • Gastric wall burn=0.4% (1 out of 279)

    Side effects (per session):

    • Abdominal pain=0.4% (1 out of 279)

    • Mild to moderate increase in body temperature=8.6% (24 out of 279; 9 in high risk group)

    • Pleural effusion=18.3% (51 out of 279; 11 in high-risk group)

    Vogl, 2004

    Local recurrence rate at 3 and 6 months by tumour size (groups 2 and 3):

    • 0 to 2 cm (n=474) = 1.3% and 1.9%

    • 2 to 3 cm (n=539) = 2.0% and 2.4%

    • 3 to 4 cm (n=327) = 1.2% at 3 and 6 months

    • More than 4 cm (n=294) = 3.7% and 4.4%

    There were no further local recurrences after 6 months.

    Mean survivalfrom date of diagnosis of metastasis for all patients=4.4 years (95% CI 4.0 to 4.8)

    • 1-year survival=94%

    • 2-year survival=77%

    • 3-year survival=56%

    • 5-year survival=37%

    Median survival=3.5 years (95% CI 3.0 to 3.9)

    Mean survival from first laser ablation=3.8 years (95% CI 3.4 to 4.2)

    • 1-year survival=86%

    • 2-year survival=64%

    • 3-year survival=49%

    • 5-year survival=33%

    Median survival=2.9 years (95% CI 2.4 to 3.3)

    Clinically relevant complications (per session):

    • Pleural effusion=1.1% (17 out of 1,555)

    • Intra-abdominal bleeding=0.1% (2 out of 1,555)

    • Liver abscess=0.4% (6 out of 1,555)

    • Death within 30 days=0.1% (2 out of 1,555); 1 patient died 4 weeks after the procedure from peritonitis and respiratory failure, after developing leakage in the jejunum. The other patient died from suspected sepsis, but this was unconfirmed.

    • Pneumothorax=0.1% (1 out of 1,555)

    • Injury to bile duct=0.1% (1 out of 1,555)

    • Bronchial-biliary fistula=0.1% (1 out of 1,555)

    No seeding of metastases along the cannulation tract was identified.

    Vogl, 2014

    Median overall survival from first laser ablation=25 months (95% CI 22.5 to 27.5)

    Mean survival=29.3 months (range 0.03 to 121)

    • 1-year overall survival=78%

    • 2-year overall survival=50.1%

    • 3-year overall survival=28%

    • 4-year overall survival=16.4%

    • 5-year overall survival=7.8%

    Median survival in those who had treatment with curative intent (n=337) = 29 months (95% CI 25.4 to 32.6); mean=32.6 (range 2 to 121)

    Median survival in those who had treatment with palliative intent (n=257) = 21 months (95% CI 18.5 to 23.5); mean 24.1 (range 0.03 to 64), p<0.001

    A multivariate Cox model identified the most significant prognostic factors for overall survival as the number (p<0.001) and diameter (p<0.001) of metastases as well as the primary surgical lymph node stage (p<0.003).

    Median progression-free survival=13 months (95% CI 11.1 to 14.9)

    Mean progression-free survival=33.7 months (range 0.03 to 54)

    • 1-year progression-free survival=51.3%

    • 2-year progression-free survival=35.4%

    • 3-year progression-free survival=30.7%

    • 4-year progression-free survival=25.4%

    • 5-year progression-free survival=22.3%

    New intrahepatic tumours were detected in 343 patients (58%) during follow up; 2% (n=12) of all patients developed a local recurrence.

    The diameter and the initial number of metastases were the most important prognostic factors for progression-free survival.

    Further treatment

    271 patients (46%) had further treatment after the final laser ablation because they showed signs of progressive disease (12% had systemic chemotherapy, 29% had TACE and 5% had both).

    No safety outcomes were reported.

    Vogl, 2013

    Median overall survival from first laser ablation= 37.6 months (range 0.03 to 1631.9)

    Mean overall survival=62.0 months (SD 25.9)

    • 1-year overall survival=86.5%

    • 2-year overall survival=67.2%

    • 3-year overall survival=51.9%

    • 4-year overall survival=39.9%

    • 5-year overall survival=33.4%

    Statistically significant prognostic factors for the long-term survival up to a very high probability: initial number of metastases (p=0.008), mean volume of metastasis (p<0.001), the quotient of the total volumes of necroses and metastases (p<0.001), and the T stage of the primary tumour according to the TNM classification (p<0.001).

    Median progression-free survival=12.2 months (range 0.03 to 133.8)

    Mean progression-free survival=35.2 months (SD 18.2)

    • 1-year progression-free survival=50.6%

    • 2-year progression-free survival=33.8%

    • 3-year progression-free survival=26%

    • 4-year progression-free survival=20.4%

    • 5-year progression-free survival=17%

    The Cox regression model identified the mean volume of metastasis (p<0.001), the quotient of total volumes (p=0.02), and the initial number of metastases (p=0.001) as statistically significant prognostic factors.

    The location of the primary tumour had no prognostic value.

    No safety outcomes were reported.

    Helmberger, 2002

    The aim of the procedure was to reduce the total tumour load rather than complete tumour destruction, to possibly qualify the patient for further selective chemotherapy.

    After the laser ablation, the patient was offered superselective chemoembolisation of the metastases. Two years later, and after 19 sessions of superselective chemoembolisation, the metastases were still under local control without signs of significant progression.

    Massive cardiac air embolism

    A spiral CT scan was done immediately after the last laser fibre was removed but removed but with the introducer sheath still in place. The scan revealed air within the right atrium, the right ventricle, and the hepatic veins. The scan was interrupted and at the same time, the patient became pulseless and unconscious. The right ventricle was percutaneously punctured with a needle. Mechanical cardiopulmonary resuscitation was not done, to prevent air embolisation into the pulmonary veins. Within 1 minute 250 ml air had been evacuated and, after external electrical defibrillation, regular heart action was re-established. The patient recovered completely within minutes and remained hemodynamically stable. After 1 hour, he was fully oriented except for an amnesia of the procedure and the subsequent 10 minutes.

    Procedure technique

    Of the 13 studies, 7 stated that the laser ablation was done under ultrasound guidance and 4 stated that the ablation was done under MRI guidance, with the needles being placed under CT guidance. Of the 7 using ultrasound, 3 stated that an Echolaser system was used, which consists of an ultrasound device and a diode laser unit (Di Costanzo 2015, Orlacchio 2014, Zou 2017). Other studies stated that a Nd:YAG laser was used. Most studies described using up to 4 fibres for ablation.

    Efficacy

    Complete tumour ablation

    The rates of complete tumour ablation were reported in 9 studies and ranged from 67% to 99%.

    In the randomised controlled trial of 140 people with HCC (157 tumours), complete tumour ablation was reported in 96% (67 out of 70; 95% CI 88 to 99) of people who had laser ablation and 97% (68 out of 70; 95% CI 90 to 99) of people who had RFA. More treatment sessions were needed in the laser group to obtain complete tumour ablation (82 compared with 72 in the RFA group, p=0.058; Di Costanzo 2015). In the randomised controlled trial of 30 people with HCC (30 tumours), a complete response at 30 days was reported in 67% (10 out of 15) of those who had laser ablation and 87% (13 out of 15) of those who had RFA. After a second treatment session, the rates were 87% (13 out of 15) in the laser ablation group and 93% (14 out of 15) in the RFA group (p=not significant; Orlacchio 2014). In the randomised controlled trial of 81 people with HCC (95 tumours), complete ablation was reported in 78% (35 out of 45) of tumours in the laser ablation group and 94% (47 out of 50) of tumours in the RFA group (Ferrari 2007). In the randomised controlled trial of 74 people with HCC, a complete response at 3 months was reported for 89% (31 out of 35) of those who had laser ablation and 92% (36 out of 39) of those who had RFA (p=0.583; Zou 2017). In the quasi-randomised controlled trial of 131 people with HCC, complete necrosis after the first treatment session was reported in 92% (46 out of 50) of tumours treated by laser ablation, 70% (14 out of 20) of tumours treated by TACE, 74% (28 out of 38) of tumours treated by PEI and 80% (32 out of 40) of tumours treated by combined TACE and PEI (Ferrari 2006). In the non-randomised comparative study of 303 people with HCC (510 tumours), initial complete ablation was reported for 99% (74 out of 75) of tumours treated by laser ablation and 98% (425 out of 435) of tumours treated by microwave ablation (Adwan 2022). In the case-control study of 82 people with a solitary HCC 40 mm or larger, a complete response was reported in 63% (21 out of 41) of those who had laser ablation and 20% (8 out of 41) of those who had TACE (p<0.001). The response rates for tumours between 51 mm and 60 mm in diameter were 75% after laser ablation and 14% after TACE (p=0.013; Morisco 2018).

    In 2 single-arm studies of people with HCC, the complete response after laser ablation was 80% (436 out of 548; Pacella 2009) and 93% (170 out of 182) of tumours (Francica 2012).

    Local tumour progression or recurrence

    Local tumour progression, progression-free survival or recurrence was reported as an outcome in 10 studies.

    In the randomised controlled trial of 140 people with HCC, local tumour progression was reported in 23% (16 out of 70) of people who had laser ablation and 26% (18 out of 70) of people who had RFA (p=0.956). The mean time to local progression was similar between the groups (46 months for laser ablation and 40 months for RFA, p=0.376; Di Costanzo 2015). In the randomised controlled trial of 30 people with HCC, cumulative rates of freedom from local disease progression at 3, 6 and 12 months after treatment were 85%, 62% and 54% in the laser ablation group, and 92%, 86% and 86% respectively in the RFA group (p=0.083; Orlacchio 2014). In the randomised controlled trial of 81 people with HCC, disease recurrence (local tumour progression or new HCC) was reported in 27% (11 out of 41) of those who had laser ablation and 23% (9 out of 40) of those who had RFA. All local tumour progression was observed within 24 months after treatment (Ferrari 2007). In the non-randomised comparative study of 303 people with HCC, local tumour progression was reported in 4% (2 out of 53) of those who had laser ablation and 6% (15 out of 250) of those who had microwave ablation. Local tumour progression-free survival at 1, 3 and 5 years from ablation date was similar between the groups (Adwan 2022). In the case control study of 82 people with a solitary HCC 40 mm or larger, the rate of disease recurrence after initially successfully treatment was 19% (5 out of 26) in the laser ablation group and 75% (6 out of 8) in the TACE group (p<0.0001; Morisco 2018).

    In the cohort study of 164 people with HCC (182 tumours), the rate of local tumour progression was 11% (18 out of 170). There was no statistically significant difference in the cumulative incidence of local tumour progression among tumours at high-risk sites compared with elsewhere (p=0.499; Francica 2012). In the cohort study of 432 people with HCC, the rate of recurrence (local and distant) was 20% (67 out of 338). The mean time to recurrence was 24 months (95% CI 20 to 28; Pacella 2009).

    In the cohort study of 603 people with CRC liver metastases, local recurrence rate at 6 months was 2% for tumours 0 cm to 2 cm in diameter, 2% for tumours between 2 cm to 3 cm, 1% for tumours between 3 cm and 4 cm and 4% for tumours larger than 4 cm in diameter (Vogl 2004). In the cohort study of 594 people with CRC liver metastases (1,545 tumours), the median progression-free survival was 13 months (95% CI 11 to 15). Progression-free survival was 51% at 1 year, 35% at 2 years, 31% at 3 years, 25% at 4 years and 22% at 5 years (Vogl 2014). In the cohort study of 401 people with liver metastases from primary sites other than CRC (809 tumours), median progression-free survival was 12 months (range 0 to 134). Progression-free survival was 51% at 1 year, 34% at 2 years, 26% at 3 years, 20% at 4 years and 17% at 5 years (Vogl 2013).

    Disease-free survival

    Disease-free survival was reported in 3 studies. In the non-randomised comparative study of 303 people with HCC, disease-free survival at 1, 3 and 5 years from ablation date was 55%, 30% and 17% in the laser ablation group, compared with 46%, 31% and 25% in the microwave ablation group (p=0.719; Adwan 2022). In the case-control study of 82 people with a solitary HCC 40 mm or larger, mean disease-free survival was 31.5 months (95% CI 24.1 to 38.8) in the laser ablation group and 14.2 months (95% CI 9.7 to 18.7) in the TACE group (p<0.0001; Morisco 2018).

    In the cohort study of 432 people with HCC, median disease-free survival time was 26 months (95% CI 22 to 30; Pacella 2009).

    Overall survival

    Overall survival was reported in 9 studies. In the randomised controlled trial of 140 people with HCC, mean overall survival was 42 months (95% CI 37 to 47) in the laser ablation group and 43 months (95% CI 38 to 47) in the RFA group (p=0.346). The 1-year survival probability was 94% in both groups and the 3-year survival probability was 80% in the laser group and 89% in the RFA group (Di Costanzo 2015). In the randomised controlled trial of 81 people with HCC, the cumulative survival rates at 1 to 5 years were higher in the RFA group than the laser ablation group, but the difference was not statistically significant (Ferrari 2007). In the quasi-randomised controlled trial of 131 people with HCC, cumulative survival at 1, 3 and 5 years was 90%, 53% and 31% for those who had treatment with laser ablation, 67%, 11% and 0% for those who had treatment with TACE, 71%, 29% and 18% for those who had treatment with PEI, and 91%, 41% and 19% for those who had treatment with combined TACE and PEI. Those who had laser ablation or combined therapy survived longer than those who had TACE (p=0.0001 and 0.0096, respectively), and laser ablation was associated with longer survival than PEI (p=0.0274; Ferrari 2006). In the non-randomised comparative study of 303 people with HCC, overall survival at 1, 3 and 5 years from diagnosis was 96%, 55% and 30% in the laser ablation group, compared with 94%, 65% and 49% in the microwave ablation group (p=0.002). Overall survival at 1, 3 and 5 years from ablation date was 85%, 38% and 17% in the laser ablation group, compared with 87%, 53% and 40% in the microwave ablation group (p=0.001; Adwan 2022). In the case control study of 82 people with a solitary HCC 40 mm or larger, mean overall survival was 40 months (95% CI 33 to 46) in the laser ablation group and 37 months (95% CI 31 to 43) in the TACE group (p=0.725). Overall survival probability at 1, 2 and 3 years was 90%, 66% and 55% in the laser group, compared with 85%, 66% and 49% in the TACE group (Morisco 2018).

    In the cohort study of 432 people with HCC (548 tumours), median overall survival was 47 months (95% CI 41 to 53), 3-year cumulative survival was 61% and 5-year cumulative survival was 34%. Survival duration was statistically significantly longer in patients with main tumour size of 2.0 cm or less compared with 3.1 cm to 4.0 cm (p=0.003), and in those with main tumour size of 2.1 cm to 3.0 cm compared with 3.1 cm to 4.0 cm (p=0.027; Pacella 2009).

    In the cohort study of 603 people with CRC liver metastases, mean survival from date of diagnosis of metastasis was 4.4 years (95% CI 4.0 to 4.8) and median survival was 3.5 years (95% CI 3.0 to 3.9). Survival at 1, 2, 3, and 5 years was 94%, 77%, 56% and 37%, respectively. Mean survival from date of first laser ablation was 3.8 years (95% CI 3.4 to 4.2) and median survival was 2.9 years (95% CI 2.4 to 3.3). Survival at 1, 2, 3, and 5 years was 86%, 64%, 49% and 33%, respectively (Vogl 2004). In the cohort study of 594 people with CRC liver metastases, the median overall survival from first laser ablation was 25 months (95% CI 22.5 to 27.5). Mean survival was 29.3 months (range 0 to 121). Overall survival was 78% at 1 year, 50% at 2 years, 28% at 3 years, 16% at 4 years and 8% at 5 years (Vogl 2014). In the cohort study of 401 people with liver metastases from primary sites other than CRC, median overall survival from first laser ablation was 37.6 months (range 0 to 1,632). Mean overall survival was 62 months (SD 25.9). Overall survival was 87% at 1 year, 67% at 2 years, 52% at 3 years, 40% at 4 years and 33% at 5 years. Statistically significant prognostic factors for long-term survival were the initial number of metastases (p=0.008), mean volume of metastasis (p<0.001), the quotient of the total volumes of necroses and metastases (p<0.001), and the T stage of the primary tumour according to the TNM classification (p<0.001; Vogl 2013).

    Patient satisfaction

    In the randomised controlled trial of 74 people with HCC, the proportion of people who reported great or general satisfaction 3 days after treatment was 94% in the laser group and 69% in the RFA group (p=0.022; Zou 2017).

    Safety

    Complications that were described as major or clinically relevant have been summarised below.

    Mortality

    One treatment-related death was reported in the cohort study of 432 people. Acute liver decompensation associated with severe respiratory failure happened 4 days after the procedure (Pacella 2009). Death within 30 days was reported after less than 1% (2 out of 1,555) of treatment sessions in the cohort study of 603 people. One person died 4 weeks after the procedure from peritonitis and respiratory failure, after developing leakage in the jejunum. The other person died from suspected sepsis, but this was unconfirmed (Vogl 2004).

    Cardiac air embolism

    A case report of massive cardiac air embolism after laser ablation was published in 2002. The air embolism was identified during the CT scan that was done immediately after the procedure. The right ventricle was percutaneously punctured with a needle and the air was evacuated. After external electrical defibrillation, the patient recovered and remained haemodynamically stable (Helmberger 2002).

    Pancreatitis

    Pancreatitis within 24 hours of laser ablation, categorised as a major complication, was reported in 1 person in the cohort study of 432 people (Pacella 2009).

    Intrahepatic haematoma

    Intrahepatic haematoma within 24 hours of laser ablation, categorised as a major complication, was reported in 1 person in the cohort study of 432 people (Pacella 2009).

    Bleeding

    Major peritoneal bleeding within 24 hours of laser ablation was reported in 1 person in the cohort study of 432 people (Pacella 2009). Clinically relevant intra-abdominal bleeding was reported after less than 1% (2 out of 1,555) of treatment sessions in the cohort study of 603 people (Vogl 2004).

    Transient decompensation of liver function

    Major transient decompensation of liver function was reported in 1% (4 out of 432) of people within 30 days of laser ablation.

    Bile duct injury

    Main bile duct injury was reported in 1 person, who had a tumour in a high-risk location, after laser ablation in the cohort study of 164 people (Francica 2012). Injury to the bile duct was reported in 1 person in the cohort study of 603 people (Vogl 2004).

    Pleural effusion

    Clinically relevant pleural effusion was reported after 1% (17 out of 1,555) of treatment sessions in the cohort study of 603 people (Vogl 2004).

    Liver abscess

    Liver abscess, described as clinically relevant, was reported after less than 1% (6 out of 1,555) treatment sessions in the cohort study of 603 people (Vogl 2004).

    Pneumothorax

    Pneumothorax, described as clinically relevant, was reported in 1 person in the cohort study of 603 people (Vogl 2004).

    Bronchial-biliary fistula

    Bronchial-biliary fistula, described as clinically relevant, was reported in 1 person in the cohort study of 603 people (Vogl 2004).

    Tumour seeding

    Subcutaneous tumour seeding was reported in 1 person who had laser ablation and 1 who had RFA in the randomised controlled trial of 140 people with HCC. In both people, it was surgically removed (Di Costanzo 2015). Three studies including 690 people who had laser ablation specified that no tumour seeding had been observed (Vogl 2004, Ferrari 2006 and 2007).

    Anecdotal and theoretical adverse events

    Expert advice was sought from consultants who have been nominated or ratified by their professional society or royal college. They were asked if they knew of any other adverse events for this procedure that they had heard about (anecdotal), which were not reported in the literature. They were also asked if they thought there were other adverse events that might possibly occur, even if they had never happened (theoretical).

    They did not describe any additional anecdotal or theoretical adverse events.

    Three professional expert questionnaires for this procedure were submitted. Find full details of what the professional experts said about the procedure in the specialist advice questionnaires for this procedure.

    Validity and generalisability

    • Most of the data included in the key evidence is from Europe.

    • There are several randomised controlled trials comparing laser ablation with RFA for treating HCC. No randomised controlled trials were identified for metastatic liver cancer.

    • In the non-inferiority randomised controlled trial by Di Costanzo (2015), the 2 treatment groups were not entirely balanced because of the random inclusion of more large and high-risk located tumours in the laser ablation group.

    • The retrospective case control study by Morisco (2018) used multicentre historical controls treated with TACE to compare with laser ablation at a single centre, which may have introduced some bias.

    • Different techniques were used to do laser ablation, including different laser systems and different types of imaging.

    • Some studies included people who had already had treatment for liver cancer, but some excluded those who had previous treatment.

    • Some studies included people who had refused surgery as well as those for whom surgery was unsuitable. The outcomes may be different in these separate groups.

    • Most studies included people with up to 5 small tumours (generally up to 50 mm in diameter). One study compared laser ablation with TACE for a solitary large HCC (up to 76 mm; Morisco 2018). The randomised controlled trial by Orlacchio (2014) only included people with a single HCC tumour with maximum diameter 40 mm. It is likely that multifocal HCC has a different biological behaviour compared with unifocal HCC, with worse trends in terms of both survival and recurrence.

    • Most studies excluded people with Child-Pugh class C liver disease.

    • Some studies reported response rates after the first treatment session, but others reported the rates after additional sessions.

    • One author noted that particularly for HCC, disease-free survival time and overall survival may be related to the aetiology and severity of the liver failure associated with cirrhosis and histological type of tumour. Survival rates can be influenced by the onset of other tumours, both primary and secondary, or complications resulting from liver failure. Local disease progression-free survival or recurrence-free survival may be a better outcome measure for local ablation procedures.

    • Several studies reported follow up of 5 years or longer.

    • No potential conflicts of interest were declared in the key evidence papers.

    • No ongoing key trials were identified.